The range of Covid 19 tests is very small. Companies that manufacture important chemicals – so-called regents – cannot meet the demand. However, researchers at the University of West Virginia are developing new tests themselves to determine who Conway 19 is now and who has had it in the past but has recovered.
Evan Martinez, associate professor at WVU, said: “It is a major problem to get enough reagents for thousands of sample tests. Not only at WVU, but also in West Virginia and not just in the United States. Worldwide.” Cancer Institute and School of Medicine. “You have to be creative if you don’t have the resources. How can we do something here at EMU so that we don’t have to rely on these companies?”
It will be important to run multiple COVID-19 tests simultaneously to test large sections of the West Virginia population or students returning to WVU in the fall semester.
“We hope that our work will help the state and WVU to reopen business quickly and safely,” said Peter Stelloff, associate professor of biochemistry.
Martinez and Stelloff are running a new diagnostic test that can detect novel coronaviruses in a swab sample.
This test detects the RNA from SARS COVID 2, the virus that causes COVID-19. The single component of the RNA nuc-nucleic acid contains all the genetic instructions that are necessary for the production of the SARS Covey 2 virus. In humans, DNA works with its well-known double helix molecular structure.
Only those who are currently infected with COVID-19 are tested positive for SARS-CoV-2 RNA. Everyone is tested negative, including people infected with another coronavirus that can usually cause a cold.
It is important to be as sensitive as possible to the test. There is always the possibility that the amount of virus is too small to be recognized. Sensitive testing reduces the likelihood that viral RNA will be “ignored” and a patient will be considered negative for CoVID-19 if they are actually infected and the disease is different. Can be transferred to people.
“This is a very important thing that everyone who experiences it should understand: we can believe that a positive test is positive, but we can never say with certainty that a negative test is negative,” Stelloff said. Is. “” We can only say that there were not enough viruses to test for this virus. It is possible that there were no viruses at all, but it is also possible that two virus particles were used to test the signal production. To be present
The tests that Stelloff, Martinez and their team are developing can detect low levels of RNA in the sample. It works by adding even small amounts of SARS-CoV-2 RNA.
“Imagine the virus that accumulates outside of Lagos,” said Martinez, who teaches microbiology, immunology, and cell biology. “We use a chemical-free buffer that separates the Lagos. This way we can expose the RNA genome of the virus. Then we use very small magnetic spheres that are bound to RNA so that we can separate them from the rest. Then we use special pieces of DNA called primers that are bound to a region of this RNA, then we use two enzymes: one that copies the RNA genome of the virus into DNA and another enzyme that copies this tiny piece of DNA several times. ”
Because DNA fragments replicate quickly – two become four, four become 16, 16 becomes 256 – fluorescence sensors are created that allow scientists to shine brighter and brighter.
“Whatever you do with the enzyme, after 30 to 40 cycles you will have trillion copies of DNA,” said Martinez. “And those trillions of copies give you trillions of signals. That’s why this test is so sensitive.”
How sensitive is it? Once the nose has been transplanted to coronavirus, the order is sent to a laboratory and placed in a viral transport medium, one milliliter of fluid, before being tested. With the WVU test, 75 blotted copies of the RNA are sufficient to disseminate the positive result if the virus is present.
“To give you an example: The test used by the CDC originally had a detection limit of a thousand copies per milliliter,” said Petrol Perotta from the Department of Pathology, Anatomy and Laboratory Medicine. Professor and Chair Development Team “This test was developed by Dars. Martinez and Stelloff are more sensitive than many of the tests currently used in this country.”
Test accuracy is not the only benefit. The number of samples can also be simultaneous.
“We are now working to fully automate the COVID-19 test